Experience in NMR
We have many years of experience in the following fields of NMR:
- NMR in solution and solid state, BioNMR, semi-solids/polymers ("HR-MAS")
- Agilent/Varian/Bruker software and hardware (setup, maintenance, repairs, worked in
Varian/Agilent application lab and service, long-time user)
- MestreNova software
- Training, courses
- Talks, lectures, publications, manuals
- Structure determination and verification of
organic molecules and proteins
- Quantification (qNMR), purity analysis
- Diffusion ("DOSY")
- Pulse sequence (C), macro (MAGICAL) and interface programming (XML)
- GMP, CFR part 11 setup (VnmrJ for Secure Environments)
- Unix/Linux system administration, shell scripts, data backup
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List of publications:
- Aleksandra Owczarek, Arkadiusz Kłys and Monika A Olszewska.
A validated 1H qNMR method for direct and simultaneous quantification
of esculin, fraxin and (-)-epicatechin in Hippocastani cortex.
Talanta 192 (2019), 263-269.
- Beata Sadowska, Łukasz Kuźma, Bartłomiej Micota, Aleksandra Budzyńska, Halina Wysokińska, Arkadiusz Kłys, Marzena Więckowska-Szakiel and Barbara Różalska.
New biological potential of abietane diterpenoids isolated from Salvia austriaca against microbial virulence factors.
Microb. Pathog. 98 (2016), 132-139.
- Magdalena Markowicz, Paweł Szymański, Marcin Ciszewski, Arkadiusz Kłys and Elżbieta Mikiciuk-Olasik.
Evaluation of poly(amidoamine) dendrimers as potential carriers of iminodiacetic derivatives using solubility
studies and 2D-NOESY NMR spectroscopy.
J. Biol. Phys. 38(4) (2012), 637-656.
- Dominika Wrobel, Arkadiusz Kłys, Maksim Ionov, Pavol Vitovic, Iveta Waczulikowa, Tibor Hianik, Rafael Gomez-Ramirez, Javier de la Mata, Barbara Klajnert and Maria Bryszewska.
Cationic carbosilane dendrimers-lipid membrane interactions.
Chem. Phys. Lipids 165(4) (2012), 401-407.
- Craig P. Butts, Bert Heise and Godiarone Tatolo.
SelEXSIDE: Fast and Easy Measurement of
Multiple-Bond 1H,
13C Coupling
Constants for Stereochemical Analysis.
Org.
Lett. 14 (2012), 3256-3259.
- Jörg Leppert, Bert Heise, Oliver
Ohlenschläger, Matthias Görlach and Ramadurai Ramachandran.
Triple resonance MAS NMR with (13C, 15N) labelled
molecules: reduced dimensionality data
acquisition via 13C-15N heteronuclear
two-spin coherence transfer pathways.
Journal
of Biomolecular NMR 28 (2004), 185-190.
- Jörg Leppert, Bert Heise, Oliver
Ohlenschläger, Matthias Görlach and Ramadurai
Ramachandran.
Broadband RFDR with adiabatic inversion pulses.
Journal
of Biomolecular NMR 26 (2003),
13-24.
- Malgorzata Palczewska, Patrick Groves, Gyula
Batta, Bert Heise and Jacek Kuznicki.
Calretinin and calbindin D28k have different
domain organizations.
Protein
Science 12 (2003), 180-184.
- Bert Heise, Jörg Leppert, Oliver
Ohlenschläger, Matthias Görlach and Ramadurai
Ramachandran.
Chemical shift correlation via RFDR:
Elimination of resonance offset effects.
Journal
of Biomolecular NMR 24 (2002),
237-243.
- Jörg Leppert, Bert Heise, Matthias Görlach
and Ramadurai Ramachandran.
REDOR: An assessment of the efficacy of
dipolar recoupling with adiabatic inversion
pulses.
Journal
of Biomolecular NMR 23 (2002),
227-238.
- Jörg Leppert, Bert Heise and Ramadurai
Ramachandran.
Orientational information from TEDOR spectral
sidebands.
Solid
State Nuclear Magnetic Resonance 19
(2001), 1-18.
- Bert Heise, Jörg Leppert, Holger Wenschuh,
Oliver Ohlenschläger, Matthias Görlach and
Ramadurai Ramachandran.
2D relayed anisotropy correlation NMR:
Characterization of the 13C
chemical shift tensor orientation in the
peptide plane of the dipeptide AibAib.
Journal
of Biomolecular NMR 19 (2001),
167-179.
- Jörg Leppert, Bert Heise and Ramadurai
Ramachandran.
REDOR in IS1S2 systems.
Journal
of Biomolecular NMR 18 (2000),
153-164.
- Bert Heise, Jörg Leppert and Ramadurai
Ramachandran.
REDOR with adiabatic dephasing pulses.
Journal
of Magnetic Resonance 146
(2000), 181-187.
- Jörg Leppert, Bert Heise and Ramadurai
Ramachandran.
15N chemical
shift tensor magnitude and orientation in the
molecular frame of uracil determined via MAS
NMR.
Journal
of Magnetic Resonance 145
(2000), 307-314.
- Bert Heise, Jörg Leppert and Ramadurai
Ramachandran.
Characterization of 15N
chemical shift tensors via 15N-13C REDOR and 15N-1H dipolar shift
CPMAS NMR spectroscopy.
Solid
State Nuclear Magnetic Resonance 16/3
(2000), 177-187.
- Roswitha Anders, Oliver Ohlenschläger, Vukic
Šoškic, Holger Wenschuh, Bert Heise and Larry
R. Brown.
The NMR solution structure of the ion channel
peptaibol chrysospermin C bound to
dodecylphosphocholine micelles.
European
Journal of Biochemistry 267(6)
(2000), 1784-1794.
- Jörg Leppert, Bert Heise and Ramadurai
Ramachandran.
Magic angle spinning NMR spectroscopy with
composite RF pulses.
Journal
of Magnetic Resonance 139
(1999), 382-388.
PhD Thesis, Bert Heise
At Fritz
Lipmann Institute for Age Research (FLI,
formerly Institute for Molecular Biotechnology,
IMB), Jena, Germany
"NMR spectroscopy on peptaibols in the solid state
and in solution: Chemical shift tensors in
α-aminoisobutyric acid and structural
investigations on Chrysospermin C". Full PDF text
(German)
Diploma Thesis
At TU
Dortmund, Germany
"7Li NMR
relaxation in Li2S-GeS2 glasses"
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